1ABS
PHOTOLYSED CARBONMONOXY-MYOGLOBIN AT 20 K
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 20 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1994-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.420, 90.420, 45.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 1.500 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 2mgk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | 1.700 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.063 | |
| Total number of observations | 93946 * | |
| Number of reflections | 30947 | |
| Completeness [%] | 90.0 | 78 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 9 | PROTEIN WAS CRYSTALLIZED FROM 3.6 M AMMONIUM SULFATE. CRYSTALS WER SOAKED FOR 1 HOUR IN A THOROUGHLY DEGASSED AND N2 - SATURATED CRYOPROTECTANT SOLUTION MADE BY ADDITION OF 100 MG SUCROSE, 100 MG GLUCOSE AND 8 MG NA-DITHIONATE TO 1 ML OF 70% SATURATED AMMONIUM SULFATE WITH 50 MM TRIS. HCL PH 9.0. THEN THE SOLUTION WAS EXCHANGED AGAINST A CO SATURATED ONE. DISTINCT COLOR CHANGES ACCOMPANIED THE FORMATION OF UNLIGATED MYOGLOBIN FROM MET-MB AND MBCO FROM UNLIGATED MB. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 70 (mg/ml) | |
| 2 | 1 | 1 | ammonium sulfate | 2.2-2.6 (M) | |
| 3 | 1 | 1 | Tris-HCl | 20 (mM) | |
| 4 | 1 | 1 | EDTA | 1 (mM) |
