1ABQ
CRYSTAL STRUCTURE OF THE UNLIGANDED ABL TYROSINE KINASE SH3 DOMAIN
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-09-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 51.400, 51.400, 46.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.060 |
| Data reduction software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.080 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.2 * | used as seeds * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 10 | 1 | drop | lyophilized 3BP-2 peptide | ||
| 2 | 1 | drop | Tris | 10 (mM) | |
| 3 | 1 | drop | 270 (mM) | ||
| 4 | 1 | reservoir | sodium citrate | 90 (mM) | |
| 5 | 1 | reservoir | Bis-Tris propane | 90 (mM) | |
| 6 | 1 | reservoir | DTT | 0.9 (mM) | |
| 7 | 1 | reservoir | EDTA | 0.9 (mM) | |
| 8 | 1 | reservoir | 0.9 (mM) | ||
| 9 | 1 | reservoir | ammonium sulfate | 38-42 (%sat) |
