1ABO
CRYSTAL STRUCTURE OF THE COMPLEX OF THE ABL TYROSINE KINASE SH3 DOMAIN WITH 3BP-1 SYNTHETIC PEPTIDE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-12-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.800, 54.200, 35.000 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.156 |
| Rwork | 0.156 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.510 |
| Data reduction software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.029 |
| Completeness [%] | 89.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.2 * | used as seeds * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 10 | 1 | drop | lyophilized 3BP-2 peptide | ||
| 2 | 1 | drop | Tris | 10 (mM) | |
| 3 | 1 | drop | 270 (mM) | ||
| 4 | 1 | reservoir | sodium citrate | 90 (mM) | |
| 5 | 1 | reservoir | Bis-Tris propane | 90 (mM) | |
| 6 | 1 | reservoir | DTT | 0.9 (mM) | |
| 7 | 1 | reservoir | EDTA | 0.9 (mM) | |
| 8 | 1 | reservoir | 0.9 (mM) | ||
| 9 | 1 | reservoir | ammonium sulfate | 38-42 (%sat) |
