1ABN
THE CRYSTAL STRUCTURE OF THE ALDOSE REDUCTASE NADPH BINARY COMPLEX
Experimental procedure
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 74.710, 85.320, 106.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.400 |
| R-factor | 0.286 |
| RMSD bond length | 0.022 * |
| RMSD bond angle | 3.850 * |
| Refinement software | CORELS |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ALR2:C298S | 10 (mg/ml) | |
| 10 | 1 | reservoir | MES | 100 (mM) | |
| 2 | 1 | drop | MES | 25 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | 0.01 (%) | ||
| 6 | 1 | drop | NADPH in H2O | 49 (mM) | |
| 7 | 1 | drop | PEG6000 | 6-13 (%) | |
| 8 | 1 | drop | MES | 25 (mM) | |
| 9 | 1 | reservoir | 0.1 (M) |
