1AB5
STRUCTURE OF CHEY MUTANT F14N, V21T
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 |
| Unit cell lengths | 54.310, 54.310, 91.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3chy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.600 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.089 | 0.350 |
| Total number of observations | 82839 * | |
| Number of reflections | 10044 | |
| <I/σ(I)> | 15.75 | |
| Completeness [%] | 92.0 | 67 |
| Redundancy | 6.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | 293 * | PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE, 2.6-3.0M IN TRIS-HCL BUFFER AT PH 7.2. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.6-3.0 (M) | |
| 2 | 1 | reservoir | Tris-HCl | ||
| 3 | 1 | drop | protein | 0.7 (mg/ml) | |
| 4 | 1 | drop | ammonium sulfate | 1.7-2.0 (M) | |
| 5 | 1 | drop | Tris-HCl |
