1AB1

SI FORM CRAMBIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	150
Detector technology	IMAGE PLATE
Collection date	1992-10
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	40.759, 18.404, 22.273
Unit cell angles	90.00, 90.70, 90.00

Refinement procedure

Resolution	10.000 - 0.890
R-factor	0.147
Structure solution method	ALREADY SOLVED
RMSD bond length	0.014
RMSD bond angle	0.033
Data reduction software	TEXSAN
Data scaling software	TEXSAN
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.670	0.897
High resolution limit [Å]	0.890	0.890
Rmerge	0.040	0.100
Number of reflections	23165
<I/σ(I)>	8.3	3.2
Completeness [%]	88.8	74.96
Redundancy	1	0.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	20 *	Teeter, M.M., (1979) J. Mol. Biol., 127, 219. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	30 (mg/ml)
2	1	drop	ethanol	80 (%(v/v))
3	1	reservoir	ethanol	50 (%)