1AA7
INFLUENZA VIRUS MATRIX PROTEIN CRYSTAL STRUCTURE AT PH 4.0
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.170, 66.170, 135.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.080 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.28000 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 18.146 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.200 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.048 | 0.025 |
| Number of reflections | 20779 | |
| <I/σ(I)> | 15 | 9 |
| Completeness [%] | 94.7 | 83 |
| Redundancy | 2.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 | 20% PEG 3350, 50MM NAH2PO4, PH 4.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 25 (mM) | |
| 3 | 1 | drop | benzamidine | 2.5 (mM) | |
| 4 | 1 | drop | 0.01 (%) | ||
| 5 | 1 | drop | PEG3350 | 10 (%) | |
| 6 | 1 | reservoir | sodium phosphate | 50 (mM) | |
| 7 | 1 | reservoir | benzamidine | 5 (mM) | |
| 8 | 1 | reservoir | 0.02 (%) | ||
| 9 | 1 | reservoir | PEG3350 | 20 (%) |
