1AA6
REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | FUJI |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 146.400, 146.400, 82.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.28700 |
| Structure solution method | MIR AND MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 24.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.085 | 0.190 |
| Total number of observations | 169244 * | |
| Number of reflections | 36025 | |
| <I/σ(I)> | 26.1 | 8.4 |
| Completeness [%] | 88.0 | 65 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.5-1.7 (M) | |
| 3 | 1 | reservoir | PEG400 | 1 (%) | |
| 4 | 1 | reservoir | sodium formate | 20 (mM) | |
| 5 | 1 | reservoir | HEPES/NaOH | 100 (mM) |
