1A9S

BOVINE PURINE NUCLEOSIDE PHOSPHORYLASE COMPLEXED WITH INOSINE AND SULFATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	296
Detector technology	AREA DETECTOR
Collection date	1996-08
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 3
Unit cell lengths	94.200, 94.200, 94.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.18
Rwork	0.180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pbn
RMSD bond length	0.006
RMSD bond angle	1.100 *
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.000
Number of reflections	15060
Completeness [%]	83.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			PROTEIN WAS CRYSTALLIZED FROM 31-35% PEG-400 IN 100 MM HEPES BUFFER, PH 7.8-8.2; 100 MM MGCL2; 1% OCTYL-BETA- D-GLUCOPYRANOSIDE. CRYSTAL SOAKED IN 12.8 MM INOSINE AND 12 MM SULFATE.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	31-35 (%)
2	1	reservoir	HEPES	100 (mM)	or Tris-HCl
3	1	reservoir	beta-D-glucopyranoside	1 (%)