1A8Y

CRYSTAL STRUCTURE OF CALSEQUESTRIN FROM RABBIT SKELETAL MUSCLE SARCOPLASMIC RETICULUM AT 2.4 A RESOLUTION

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-03
Detector	RIGAKU RAXIS II
Spacegroup name	C 2 2 21
Unit cell lengths	59.740, 145.560, 111.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.188
Rwork	0.188
R-free	0.24200
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	25.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.050	0.045
Number of reflections	14812
<I/σ(I)>	11.9	4.6
Completeness [%]	80.1	61.7
Redundancy	3.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	4 *	pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	MPD	10 (%(v/v))
3	1	reservoir	Na3 citrate	0.1 (M)
4	1	reservoir	Na cacodylate	0.05 (M)