1A8R

GTP CYCLOHYDROLASE I (H112S MUTANT) IN COMPLEX WITH GTP

Experimental procedure

Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-07-16
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	314.950, 219.140, 131.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.2
Rwork	0.200
R-free	0.24600
RMSD bond length	0.008
RMSD bond angle	1.900
Data reduction software	MOSFLM
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.085
Total number of observations	661363 *
Number of reflections	238566
Completeness [%]	90.4	91.4
Redundancy	3.1	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium MES	0.1 (M)
2	1	reservoir	sodium acetate	0.2 (M)
3	1	reservoir	sodium azide	3 (mM)