1A87

COLICIN N

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1990-06
Detector	MARRESEARCH
Spacegroup name	I 41 3 2
Unit cell lengths	187.300, 187.300, 187.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.000 - 3.100
R-factor	0.173 *
Rwork	0.173
R-free	0.31000
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.000 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	GROPAT
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.700	3.200
High resolution limit [Å]	3.100	3.100
Rmerge	0.083 *
Number of reflections	10644
<I/σ(I)>	33.07	6.3
Completeness [%]	93.5	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	phosphate	50 (mM)
3	1	reservoir	ammonium sulfate	60 (%(w/v)sat)