1A7W

CRYSTAL STRUCTURE OF THE HISTONE HMFB FROM METHANOTHERMUS FERVIDUS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-02
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 2
Unit cell lengths	31.300, 61.580, 39.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 - 1.900 *
R-factor	0.18 *
Rwork	0.198
R-free	0.22900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PARTIALLY REFINED ORTHORHOMBIC HMFA
RMSD bond length	0.021 *
RMSD bond angle	0.037 *
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.100	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.053	0.196
Total number of observations	25200 *
Number of reflections	6152
<I/σ(I)>	8.3	3.7
Completeness [%]	95.9	97.2
Redundancy	4.1	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	potassium citrate	100 (mM)
3	1	reservoir	Tris-HCl	50 (mM)