1A7V

CYTOCHROME C' FROM RHODOPSEUDOMONAS PALUSTRIS

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-07
Spacegroup name	P 21 21 21
Unit cell lengths	63.000, 113.500, 34.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.194
Rwork	0.194
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ccy
RMSD bond length	0.012
RMSD bond angle	19.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.072	0.160
Total number of observations	39136 *
Number of reflections	10513
<I/σ(I)>	15.9	2.1
Completeness [%]	89.1	62.7 *
Redundancy	3.7	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6		drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	MES	100 (mM)
3	1	reservoir	PEG6000	30 (%(w/v))