1A7S
ATOMIC RESOLUTION STRUCTURE OF HBP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.220, 65.160, 101.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.120 |
| R-factor | 0.159 |
| R-free | 0.18900 |
| Structure solution method | PHASES FROM NATIVE HBP |
| RMSD bond length | 0.027 |
| RMSD bond angle | 0.046 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-96 |
| Refinement software | SHELXL-96 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.140 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.058 | 0.479 |
| Number of reflections | 90539 | 4055 * |
| <I/σ(I)> | 7.13 | 1.96 |
| Completeness [%] | 90.5 | 81.9 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ethanol | 14 (%) | |
| 2 | 1 | reservoir | 1,2,6-trihydroxyhexane | 5 (%) | |
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
