1A6Y
REVERBA ORPHAN NUCLEAR RECEPTOR/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.670, 45.690, 47.950 |
| Unit cell angles | 75.16, 80.64, 85.58 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.1921 * |
| Rwork | 0.192 |
| R-free | 0.28820 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2NLL (TR PORTION) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 22.080 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.84) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 * | |
| Number of reflections | 13482 | |
| <I/σ(I)> | 15.1 | 2.8 |
| Completeness [%] | 97.3 | 94 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 17 * | Zhao, Q., (1998) Mol. Cell, 1, 849. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NACL | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | TRIS | ||
| 4 | 1 | 1 | PEG 8000 | ||
| 5 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.6 (mM) | |
| 2 | 1 | drop | protein | 1.2 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 20-25 (%) | |
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | 400 (mM) | ||
| 6 | 1 | reservoir | Tris | pH7.5 |
