1A6P
ENGINEERING OF A MISFOLDED FORM OF CD2
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 41.090, 41.090, 105.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.080 |
| R-factor | 0.2186 |
| Rwork | 0.219 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hng |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.291 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.080 |
| Rmerge | 0.059 | 0.295 |
| Number of reflections | 10589 | |
| <I/σ(I)> | 16.1 | 2.57 |
| Completeness [%] | 99.1 | 99 |
| Redundancy | 2.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 20% PEG 4000, 5% GLYCEROL, 0.1M NACO, PH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | glycerol | 5 (%) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) |
