1A6L

T14C MUTANT OF AZOTOBACTER VINELANDII FDI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	291
Detector technology	AREA DETECTOR
Collection date	1998-01
Detector	SIEMENS
Spacegroup name	P 41 21 2
Unit cell lengths	55.880, 55.880, 96.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.206
Rwork	0.206
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fd1
RMSD bond length	0.014
RMSD bond angle	1.480 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.100	2.100
Rmerge	0.059 *	0.218
Total number of observations	33677 *
Number of reflections	8686
<I/σ(I)>	18.8	2.7
Completeness [%]	92.5 *	59.9 *
Redundancy	3.9	2.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4 *		pH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-10 (mg/ml)
2	1	drop	potassium phosphate	0.5 (M)
3	1	drop	ammonium sulfate	1.2 (M)
4	1	reservoir	ammonium sulfate	3.5 (M)