1A6J
NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-15 |
| Detector | MACSCIENCE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 107.900, 107.900, 64.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.350 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.25100 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.580 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.050 | 0.200 * |
| Total number of observations | 104938 * | |
| Number of reflections | 14482 | |
| <I/σ(I)> | 14 | 5 |
| Completeness [%] | 89.0 | 75 |
| Redundancy | 5.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | PROTEIN CRYSTALLIZED WITH THE HANGING DROP SETUP. RESERVOIR CONTAINING 1.8 M AMMONIUM SULPHATE, 1 MM SODIUM AZIDE, 2MM DTT AND 0.1 M BES-NAOH, PH 7.5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.9-1.0 (M) | |
| 3 | 1 | drop | 0.5 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | Bes-NaOH | 0.05 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.8-2.0 (M) | |
| 7 | 1 | reservoir | 1 (mM) | ||
| 8 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 9 | 1 | reservoir | Bes-NaOH | 0.1 (M) |
