1A6I
TET REPRESSOR, CLASS D VARIANT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 69.810, 69.810, 184.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.500 - 2.400 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tct |
| RMSD bond length | 0.007 |
| RMSD bond angle | 19.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.500 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 * | 0.248 * |
| Number of reflections | 9076 | |
| <I/σ(I)> | 6.13 | 2.9 |
| Completeness [%] | 97.8 | 98.6 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 18 * | 0.01ml protein solution was mixed with 0.005ml reservoir * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | potassium phosphate | 0.8 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) |
