1A6C
STRUCTURE OF TOBACCO RINGSPOT VIRUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-04 |
| Detector | FUJI |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 407.060, 399.680, 285.970 |
| Unit cell angles | 90.00, 129.10, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.500 |
| R-factor | 0.269 |
| Rwork | 0.269 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE OF COMOVIRUS CPMV |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.000 |
| Data reduction software | PURDUE ((OSC)) |
| Data scaling software | PROGRAMS (DEVELOPED BY M. G. ROSSMANN AT PURDUE UNIVERSITY) |
| Phasing software | RAVE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.000 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.084 | |
| Number of reflections | 92395 | |
| Completeness [%] | 18.0 | 10 |
| Redundancy | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | VIRUS WAS CRYSTALLIZED USING HANGING DROP SETTING FROM RESERVOIR BUFFER CONTAINING 2-3% (W/V) PEG 3350, 1MM SODIUM AZIDE AND 0.125 M POTASSIUM PHOSPHATE, PH 6.5, pH 6.0, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG MW 3350 | 2-3 (%) |
