1A5Q
P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-05-08 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.000, 65.000, 65.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.27300 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.310 * |
| Data reduction software | SDMS |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.380 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.134 * | 0.391 * |
| Number of reflections | 7204 | |
| <I/σ(I)> | 16.5 | 4 |
| Completeness [%] | 97.0 | 100 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | macro seeding * | 5.7 * | PROTEIN WAS CRYSTALLIZED FROM 100 MM HEPES PH 7.0, 2.2 M CSCL, AND 1.2 M AMM SO4. THEN IT WAS SOAKED IN 100 MM HEPES PH 7.0, 80% AMM SO4 FOR 24 HOURS TO REMOVE THE CS IONS., pH 6.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNase1-118 | 0.95 (mM) | |
| 2 | 1 | drop | RNase111-124 | 9.9 (mM) | |
| 3 | 1 | reservoir | 3.0 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 28 (%sat) | |
| 5 | 1 | reservoir | ammonium acetate | 0.1 (M) | |
| 6 | 1 | reservoir | 1,10-phenanthroline | 5 (mM) |
