1A5Q

P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1997-05-08
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 32 2 1
Unit cell lengths	65.000, 65.000, 65.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.211
Rwork	0.211
R-free	0.27300
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.011
RMSD bond angle	1.310 *
Data reduction software	SDMS
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.134 *	0.391 *
Number of reflections	7204
<I/σ(I)>	16.5	4
Completeness [%]	97.0	100
Redundancy	5	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	macro seeding *	5.7 *		PROTEIN WAS CRYSTALLIZED FROM 100 MM HEPES PH 7.0, 2.2 M CSCL, AND 1.2 M AMM SO4. THEN IT WAS SOAKED IN 100 MM HEPES PH 7.0, 80% AMM SO4 FOR 24 HOURS TO REMOVE THE CS IONS., pH 6.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNase1-118	0.95 (mM)
2	1	drop	RNase111-124	9.9 (mM)
3	1	reservoir		3.0 (mM)
4	1	reservoir	ammonium sulfate	28 (%sat)
5	1	reservoir	ammonium acetate	0.1 (M)
6	1	reservoir	1,10-phenanthroline	5 (mM)