1A5I
CATALYTIC DOMAIN OF VAMPIRE BAT (DESMODUS ROTUNDUS) SALIVA PLASMINOGEN ACTIVATOR IN COMPLEX WITH EGR-CMK (GLU-GLY-ARG CHLOROMETHYL KETONE)
Experimental procedure
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.860, 73.860, 135.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.900 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.930 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.092 | 0.405 |
| Total number of observations | 22337 * | |
| Number of reflections | 9582 | |
| <I/σ(I)> | 3.51 | 1.9 |
| Completeness [%] | 97.2 | 97.9 |
| Redundancy | 2.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 9 | 20 * | pH 9.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 10 (%) | |
| 2 | 1 | reservoir | Mes/Tris | 0.1 (M) |
