1A5F

FAB FRAGMENT OF A MONOCLONAL ANTI-E-SELECTIN ANTIBODY

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1995-05
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	44.460, 83.790, 132.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.800
R-factor	0.195
Rwork	0.195
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bbd
RMSD bond length	0.015
RMSD bond angle	28.200 *
Data reduction software	SCALEK ((XENGEN))
Data scaling software	SCALEK ((XENGEN))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.800
Rmerge	0.069 *
Total number of observations	42928 *
Number of reflections	10993
<I/σ(I)>	4.6
Completeness [%]	86.5
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9.6 (mg/ml)
2	1	drop	HEPES-HCl	10 (mM)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)
5	1	reservoir	PEG8000	20 (%(w/v))