1A5D

GAMMAE CRYSTALLIN FROM RAT LENS

Experimental procedure

Source details	BRUKER NONIUS
Temperature [K]	300
Detector technology	DIFFRACTOMETER
Collection date	1989-06
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 1 21 1
Unit cell lengths	37.760, 43.710, 107.440
Unit cell angles	90.00, 108.54, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.168
Rwork	0.168
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2GCR
RMSD bond length	0.009 *
RMSD bond angle	1.910 *
Data reduction software	MADNES
Data scaling software	PRECURSOR (TO ABSURD/ ROTAVATA/AGROVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.900
High resolution limit [Å]	2.300
Rmerge	0.082
Total number of observations	41615 *
Number of reflections	12942
Completeness [%]	84.7
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.86 *		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein		6.2mg
2	1	drop	sodium phosphate	0.05 (M)
3	1	drop	sodium azide	0.02 (%)
4	1	drop	dithiothreitol	3 (mM)
5	1	reservoir	PEG6000	13 (%(w/v))
6	1	reservoir	sodium phosphate	0.05 (M)