1A4V
ALPHA-LACTALBUMIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-01 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 35.470, 69.560, 45.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.26300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.400 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 * | 0.018 * |
| Total number of observations | 40180 * | |
| Number of reflections | 9928 | |
| <I/σ(I)> | 14.33 | 1.03 |
| Completeness [%] | 82.0 | 64 |
| Redundancy | 4.1 | 0.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | PIPES | 0.1 (M) | |
| 3 | 1 | drop | ammonium sulfate | 1.8 (M) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | reservoir | 100 (mM) | ||
| 7 | 1 | reservoir | PIPES | 0.2 (M) |
