1A4Q
INFLUENZA VIRUS B/BEIJING/1/87 NEURAMINIDASE COMPLEXED WITH DIHYDROPYRAN-PHENETHYL-PROPYL-CARBOXAMIDE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.300, 88.300, 221.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.000 - 1.900 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1nsc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 27.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.092 |
| Total number of observations | 208669 * |
| Number of reflections | 77084 * |
| Completeness [%] | 97.4 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | seeding * | 7.8 | pH 7.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 7-10 (%) | |
| 2 | 1 | 1 | Tris-HCl | 100 (mM) |
