1A48
SAICAR SYNTHASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | X31 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1991-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.600, 63.700, 81.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.153 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.041 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.170 |
| Number of reflections | 24879 | |
| <I/σ(I)> | 26 | 7 |
| Completeness [%] | 97.0 | 95 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8-15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | aspartic acid | 40 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 1.0-1.25 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 2.25 (M) |
