1A44
PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN FROM CALF BRAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Collection date | 1997-11 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.700, 33.550, 60.070 |
| Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
| Resolution | 32.000 * - 1.840 |
| R-factor | 0.147 * |
| Rwork | 0.148 |
| R-free | 0.21200 * |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.027 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.040 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.039 * | 0.095 * |
| Total number of observations | 48585 * | |
| Number of reflections | 14517 | |
| <I/σ(I)> | 15.8 | 8 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 20-25 (%) | |
| 5 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 6 | 1 | reservoir | acetate | 0.1 (M) |
