1A44
PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN FROM CALF BRAIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 100 |
Collection date | 1997-11 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.700, 33.550, 60.070 |
Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
Resolution | 32.000 * - 1.840 |
R-factor | 0.147 * |
Rwork | 0.148 |
R-free | 0.21200 * |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.027 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.040 | 1.940 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.039 * | 0.095 * |
Total number of observations | 48585 * | |
Number of reflections | 14517 | |
<I/σ(I)> | 15.8 | 8 |
Completeness [%] | 99.8 | 98.6 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | drop | dithiothreitol | 2 (mM) | |
4 | 1 | reservoir | PEG4000 | 20-25 (%) | |
5 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
6 | 1 | reservoir | acetate | 0.1 (M) |