1A42
HUMAN CARBONIC ANHYDRASE II COMPLEXED WITH BRINZOLAMIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-09 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.700, 41.700, 73.000 |
| Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.250 |
| R-factor | 0.144 * |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | HUMAN CAII |
| RMSD bond length | 0.013 |
| RMSD bond angle | 26.200 * |
| Data reduction software | BUDDHA |
| Data scaling software | PROTEIN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.250 |
| Rmerge | 0.098 |
| Total number of observations | 18107 * |
| Number of reflections | 8703 |
| Completeness [%] | 65.5 |
| Redundancy | 2.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 8.5 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 2.4 (M) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 3 | 1 | 1 | 1 (mM) |
