1A3S
HUMAN UBC9
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08 |
| Detector | BRUKER NONIUS |
| Spacegroup name | P 43 |
| Unit cell lengths | 73.900, 73.900, 42.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 * - 2.800 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1AAK |
| RMSD bond length | 0.011 |
| RMSD bond angle | 22.060 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.198 * |
| Number of reflections | 5605 | 474 * |
| <I/σ(I)> | 7.2 | 2.4 |
| Completeness [%] | 96.0 | 81.4 * |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.25 * | 293.5 * | pH 7. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 20 (mM) | |
| 4 | 1 | drop | sodium phosphate | 1 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | |
| 6 | 1 | reservoir | lithium sulfate | 200 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) |
