1A34
SATELLITE TOBACCO MOSAIC VIRUS/RNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-02 |
| Detector | SDMS |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 174.270, 191.770, 202.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.810 |
| R-factor | 0.179 * |
| Rwork | 0.179 |
| R-free | 0.18400 |
| Structure solution method | MIR, MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODIFIED STNV |
| RMSD bond length | 0.009 |
| RMSD bond angle | 17.770 * |
| Data reduction software | SDMS (DETECTOR SYSTEM) |
| Data scaling software | SDMS (DETECTOR SYSTEM) |
| Phasing software | HEAVY |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | 1.940 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.091 | |
| Total number of observations | 2239616 * | |
| Number of reflections | 271689 | |
| Completeness [%] | 89.0 | 63.9 |
| Redundancy | 8.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | Koszelak, S., (1989) J.Mol.Biol., 209, 323. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | AMMONIUM SULFATE | ||
| 3 | 1 | 2 | WATER | ||
| 4 | 1 | AMMONIUM SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 5-8 (mg/ml) | |
| 2 | 1 | drop | cacodylate | 10 (mM) | can be replaced by phosphate or Tris buffer |
| 3 | 1 | drop | reservoir | 0.010ml | |
| 4 | 1 | reservoir | ammonium sulfate | 10-20 (%sat) | |
| 5 | 1 | reservoir | cacodylate | 40 (mM) |
