1A1S

ORNITHINE CARBAMOYLTRANSFERASE FROM PYROCOCCUS FURIOSUS

Experimental procedure

Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1997-02
Detector	MACSCIENCE
Spacegroup name	F 2 3
Unit cell lengths	186.800, 186.800, 186.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.226
Rwork	0.226
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.090 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.780
High resolution limit [Å]	2.700	2.700
Rmerge	0.064	0.064
Number of reflections	14893
<I/σ(I)>	12	3
Completeness [%]	99.3	99.8
Redundancy	12.4	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4	21 *	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir		1 (M)
3	1	reservoir	acetate	100 (mM)