1A18
PHENANTHROLINE MODIFIED MURINE ADIPOCYTE LIPID BINDING PROTEIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-06 |
| Detector | SIEMENS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.020, 97.150, 49.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lib |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.487 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.520 |
| High resolution limit [Å] | 2.400 | 2.370 |
| Rmerge | 0.079 * | 0.325 * |
| Total number of observations | 34268 * | |
| Number of reflections | 7640 | |
| <I/σ(I)> | 13.1 | 2.3 |
| Completeness [%] | 91.7 | 62.4 |
| Redundancy | 4.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 28-31 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.1-0.25 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
