1A12

REGULATOR OF CHROMOSOME CONDENSATION (RCC1) OF HUMAN

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Collection date	1997-05
Spacegroup name	P 1
Unit cell lengths	48.957, 82.903, 83.105
Unit cell angles	112.88, 104.09, 103.42

Refinement procedure

Resolution	41.000 - 1.700
R-factor	0.189
Rwork	0.189
R-free	0.21900
Structure solution method	SINGLE ISOMORPHOUS REPLACEMENT (SIR) COMBINED WITH 3-FOLD NCS AVERAGING
RMSD bond length	0.015
RMSD bond angle	26.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.500	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.083	0.257
Total number of observations	596594 *
Number of reflections	113006
<I/σ(I)>	23.1	2.8
Completeness [%]	94.3	69.9
Redundancy	5.3	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.9		pH 6.9