1A0P
SITE-SPECIFIC RECOMBINASE, XERD
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 102.950, 102.950, 55.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.224 * |
| Rwork | 0.224 |
| R-free | 0.28700 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.041 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.048 * | |
| Number of reflections | 11671 | |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.25-2.5 (mg/ml) | |
| 2 | 1 | drop | Tris | 55 (mM) | |
| 3 | 1 | drop | 250 (mM) | can be replaced by KCl | |
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 0.5 (mM) | |
| 6 | 1 | drop | PEG400 | 3-7 (%) | |
| 7 | 1 | reservoir | Tris | 100 (mM) | |
| 8 | 1 | reservoir | PEG400 | 6-14 (%) |
