198L

THERMODYNAMIC AND STRUCTURAL COMPENSATION IN "SIZE-SWITCH" CORE-REPACKING VARIANTS OF T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	rotating-anode X-ray tube
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	AREA DETECTOR
Spacegroup name	P 32 2 1
Unit cell lengths	61.100, 61.100, 96.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.158
RMSD bond length	0.013 *
RMSD bond angle	1.900 *
Data reduction software	XUONG-HAMLIN ((DETECTOR SYSTEM))
Data scaling software	Xengen (HOWARD, NIELSEN, XUONG)
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.041
Number of reflections	12672 *
Completeness [%]	84.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6.7	4 *	pH 6.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1		0.55 (M)
3	1	1	mercaptoethanol	14 (mM)
4	1	1		1 (mM)
5	1	1	sodium phospahte	0.01 (M)	m
6	1	1		2.2 (M)
7	1	1		1.8 (M)