197L
THERMODYNAMIC AND STRUCTURAL COMPENSATION IN "SIZE-SWITCH" CORE-REPACKING VARIANTS OF T4 LYSOZYME
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.200, 61.200, 95.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 2.100 |
| R-factor | 0.163 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.000 |
| Data reduction software | XUONG-HAMLIN ((DETECTOR SYSTEM)) |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 5.000 * |
| High resolution limit [Å] | 2.100 * |
| Rmerge | 0.042 |
| Number of reflections | 10369 * |
| Completeness [%] | 87.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.7 | 4 * | pH 6.7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | 0.55 (M) | ||
| 3 | 1 | 1 | mercaptoethanol | 14 (mM) | |
| 4 | 1 | 1 | 1 (mM) | ||
| 5 | 1 | 1 | sodium phospahte | 0.01 (M) | |
| 6 | 1 | 1 | 2.2 (M) | ||
| 7 | 1 | 1 | 1.8 (M) |
