194D
X-RAY STRUCTURES OF THE B-DNA DODECAMER D(CGCGTTAACGCG) WITH AN INVERTED CENTRAL TETRANUCLEOTIDE AND ITS NETROPSIN COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 258 |
| Detector technology | AREA DETECTOR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.700, 40.500, 67.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.148 |
| Rwork | 0.148 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 28.200 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.060 * |
| Number of reflections | 2214 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 282 | pH 7.50, VAPOR DIFFUSION, temperature 282.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | ||
| 2 | 1 | drop | oligonucleotide | 1 equivalent | |
| 3 | 1 | drop | 2.5 equivalents | ||
| 4 | 1 | drop | spermine tetrachloride | 2 equivalents | |
| 5 | 1 | drop | MPD | 40 (%) | |
| 6 | 1 | reservoir | 50 (%) |
