192L

A HELIX INITIATION SIGNAL IN T4 LYSOZYME IDENTIFIED BY POLYALANINE MUTAGENESIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	298
Detector technology	FILM
Detector	OSCILLATION CAMERA
Spacegroup name	P 32 2 1
Unit cell lengths	61.100, 61.100, 95.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.169
RMSD bond length	0.016
RMSD bond angle	2.170
Data scaling software	AGROVATA / ROTAVATA
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.076 *
Completeness [%]	66.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *			A.E. Eriksson, (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol