189L

ENHANCEMENT OF PROTEIN STABILITY BY THE COMBINATION OF POINT MUTATIONS IN T4 LYSOZYME IS ADDITIVE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	rotating-anode X-ray tube
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	AREA DETECTOR
Spacegroup name	P 21 21 21
Unit cell lengths	34.600, 56.800, 94.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.2
RMSD bond length	0.018 *
RMSD bond angle	2.800 *
Data scaling software	Xengen (HOWARD, NIELSEN, XUONG)
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.046 *
Number of reflections	6024 *
Completeness [%]	88.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	20 (%)
2	1	1	tert-butyl alcohol	5 (%)
3	1	1	potassium phosphate	100 (mM)