182D
DNA-NOGALAMYCIN INTERACTIONS: THE CRYSTAL STRUCTURE OF D(TGATCA) COMPLEXED WITH NOGALAMYCIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | HENDRIX-LENTFER |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 37.200, 37.200, 70.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.195 |
| R-free | 0.30000 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.076 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.057 * |
| Total number of observations | 4788 * |
| Number of reflections | 4953 |
| Completeness [%] | 81.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 4 * | pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 0.6 (mM) | |
| 2 | 1 | drop | nogalamycin | 2.3 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 4 | 1 | drop | magnesium chloride | 8 (mM) | |
| 5 | 1 | drop | MPD | 10-20 (%) | |
| 6 | 1 | drop | spermine | 0.6 (mM) | |
| 7 | 1 | reservoir | 100 (%) |
