177L

Protein flexibility and adaptability seen in 25 crystal forms of T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1993-12-29
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 42 2 2
Unit cell lengths	72.600, 72.600, 82.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.228
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.400
Data reduction software	Xuong-Hamlin
Data scaling software	Rotovata/Agrovata
Phasing software	MRX
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500
Number of reflections	7778
Completeness [%]	97.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.6		pH 8.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	reservoir		0.1 (M)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir	reduced beta-mercaptoethanol	50 (mM)