176L
PROTEIN FLEXIBILITY AND ADAPTABILITY SEEN IN 25 CRYSTAL FORMS OF T4 LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | rotating-anode X-ray tube |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | AREA DETECTOR |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.600, 127.100, 29.100 |
| Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
| Resolution | 14.000 - 2.200 |
| R-factor | 0.183 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.300 |
| Data scaling software | Xengen (HOWARD, NIELSEN, XUONG) |
| Refinement software | TNT |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
| 2 | 1 | reservoir | 0.19 (M) | ||
| 3 | 1 | reservoir | citrate | 0.1 (M) |
