173L

PROTEIN FLEXIBILITY AND ADAPTABILITY SEEN IN 25 CRYSTAL FORMS OF T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	rotating-anode X-ray tube
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	AREA DETECTOR
Spacegroup name	P 65
Unit cell lengths	75.100, 75.100, 54.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.183
RMSD bond length	0.018
RMSD bond angle	2.300
Data scaling software	Xengen (HOWARD, NIELSEN, XUONG)
Refinement software	TNT

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	23 (%(w/v))
2	1	reservoir	sodium potassium phosphate	0.08 (M)
3	1	reservoir	oxidized beta-mercaptoethanol	70 (mM)
4	1	reservoir		0.3 (M)