167D
THE CRYSTAL STRUCTURE OF C-C-A-T-T-A-A-T-G-G: IMPLICATIONS FOR BENDING OF B-DNA AT T-A STEPS
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 33.200, 33.200, 96.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.2 |
| RMSD bond length | 0.035 |
| RMSD bond angle | 0.090 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.024 * |
| Number of reflections | 2726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion standing drops * | 4 * | VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.39 (mM) | |
| 2 | 1 | drop | 140 (mM) | ||
| 3 | 1 | drop | MPD | 15 (%(v/v)) | |
| 4 | 1 | reservoir | MPD | 35 (%) |
