15AA

Crystal structure of FtsZ with no ligand modelled

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 65
Unit cell lengths	88.292, 88.292, 178.309
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.190 - 2.310
R-factor	0.18831
Rwork	0.186
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.146	2.348
High resolution limit [Å]	2.308	2.308
Rmerge	0.251	1.953
Rmeas	0.264	2.075
Rpim	0.078	0.661
Total number of observations	367541	14150
Number of reflections	34474	1719
<I/σ(I)>	6.9
Completeness [%]	99.9
Redundancy	10.7	8.2
CC(1/2)	0.992	0.351

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-2	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.