14ZZ

Crystal Structure of FtsZ with Z2692078340

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	87.828, 87.828, 176.239
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.953 - 2.497
Rwork	0.169
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.553
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.914	2.540
High resolution limit [Å]	2.497	2.497
Rmerge	0.355	2.894
Rmeas	0.369	3.002
Rpim	0.100	0.794
Total number of observations	362246	18374
Number of reflections	26663	1309
<I/σ(I)>	6.1
Completeness [%]	100.0
Redundancy	13.6	14
CC(1/2)	0.992	0.335

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-976	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.