14ZY

Crystal Structure of FtsZ with Z57326966

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 65
Unit cell lengths	87.530, 87.530, 174.694
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.803 - 2.802
Rwork	0.167
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.537
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.765	2.850
High resolution limit [Å]	2.802	2.802
Rmerge	0.401	2.130
Rmeas	0.417	2.212
Rpim	0.114	0.597
Total number of observations	248296	12580
Number of reflections	18615	918
<I/σ(I)>	6.1
Completeness [%]	100.0
Redundancy	13.3	13.7
CC(1/2)	0.988	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-957	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.