14ZW

Crystal Structure of FtsZ with Z228587394

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 65
Unit cell lengths	88.464, 88.464, 179.604
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.271 - 2.091
Rwork	0.192
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.641
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.232	2.127
High resolution limit [Å]	2.091	2.091
Rmerge	0.265	1.948
Rmeas	0.276	2.035
Rpim	0.076	0.585
Total number of observations	624702	28378
Number of reflections	46888	2350
<I/σ(I)>	7.9
Completeness [%]	100.0
Redundancy	13.3	12.1
CC(1/2)	0.995	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-935	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.